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This data release includes metadata and tabular data that documents counts of arthropods collected from forest litter at the Liko Nā Pilina study site located on the Keaukaha Military Reservation, Hilo, Hawai‘i. These data are represented in three files: 1) a description of the date and location for each sample, 2) classification for all taxa, and 3) counts of each taxon in each sample. The classification file is in lieu of listing all taxa in the Taxonomic Classification section of the metadata files. Arthropods were extracted from litterbags containing leaves from dominant plant species within the study site and from litter collected in randomly placed quadrats. Samples were obtained after 1, 2, 4 and 6 months during 28 August 2019 - 28 January 2020. Samples were processed in the lab during 1 March 2020 - 31 December 2022, and taxa identified during 1 April - 31 December 2023. Quantitative data are only included for the litterbag samples. Overall, 319 arthropod taxa are included in this data release.more » « less
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Roy, Kylle; Jaenecke, Kelly A.; Bjontegard, Nikko; Mikros, Dan; Dunkle, Ellen J.; Yanger, Corie; Sugiyama, Lionel S.; Keith, Lisa M.; Peck, Robert W. (, Plant Health Progress)null (Ed.)Rapid ʻōhiʻa death (ROD) is caused by two recently described species of Ceratocystis, C. lukuohia and C. huliohia. These fungi are decimating ʻōhiʻa lehua (Metrosideros polymorpha), the keystone native tree species of Hawaiʻi. Viable Ceratocystis propagules can persist in ambrosia beetle frass (Coleoptera: Scolytinae), and movement of the frass may play a key role in the spread of the disease. In order to prevent the spread of ROD, we developed effective and practical surface (e.g., tools and shoes) decontamination methods to be used by researchers, managers, and the public alike. We first tested different household and laboratory disinfectants on the Ceratocystis fungi in culture, and then we applied the effective culture disinfectants to contaminated ambrosia beetle frass. Laboratory-grade ethanol (70, 80, and 95%), Clorox bleach (10%, 0.825% active ingredient [a.i.]), and isopropanol (70 and 91%), were all equally effective at decontaminating cultured C. lukuohia and C. huliohia. Although all concentrations of isopropanol (50, 70, and 90%) and ethanol (50, 70, and 90%) were effective disinfectants of Ceratocystis-contaminated frass, treatments of frass with up to 20% Clorox bleach (1.2% a.i.) were not completely adequate at killing the fungus. These data reveal that bleach is not a sufficient ROD disinfectant when frass is present, and isopropanol or ethanol are the more reliable options.more » « less
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